Project description:4-week-old male mice were infected with 50,000 CL+luc parasites or remained uninfected. Urine was collected pre-infection and 4 days after infection. 7 days post infection, mice were treated with 0.06% carnitine in drinking water ad libitum or with benznidazole 100 mg/kg ip for 10 days. 17 days post infection, urine was collected and euthanized. Metabolites were extracted from urine in methanol and untargeted LCMS analysis ran on a Q Exactive plus instrument using a Phenomenex Luna Omega Polar C18 column.
Project description:ddMS2 run of mouse lung tissue and plasma extract using C8 column in 7.5-minute gradient and positive polarity mode in Q Exactive plus.
Project description:Plasma and Cerebrospinal fluid data were acquired on the Q-Exactive with 3 different columns: a C8 column, a C18 column, and a polar C18 Column.
Project description:We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa cell protein extract, we carried out 16 different chromatography conditions on four different instrumentation platforms with three replicates of each condition, for a total of 48 proteomics analyses. We first compared selected chromatography conditions of the QE+ and OFL by varying column lengths, inner diameters, and C18-RP particle sizes. We next selected one chromatography condition on each system and varied the effective RP gradients lengths
Project description:MS/MS fragmentation data of sample VD_C13 acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of foodbiomarker standards acquired on Q Exactive - with
chromatographic separation on a Phenomenex polar C18 column.
Project description:MS/MS fragmentation data of n acyl lipids acquired on Q Exactive - with chromatographic separation on a Phenomenex polar C18 column.