Project description:ddMS2 run of mouse lung tissue and plasma extract using C8 column in 7.5-minute gradient and positive polarity mode in Q Exactive plus.
Project description:apply C8 column and 7.5 minutes run to feces, small intestine and large intestine samples from mice under negative polarity mode in ddMS2.
Project description:ddMS2 in positive polarity for swab recovery of chemicals from desk top after sampling different concentrations using PRM in Q Exactive Plus.
Project description:ddMS2 data of C2C12 treated with Carnitine, untreated C2C12, water and carnitine in water using polar C18 column in Q Exactive Plus
Project description:This study aimed to use a lamb model of preterm birth to investigate the impact of different tidal volume strategies, applied during positive pressure ventilation, on lung injury development. Preterm lambs were ventilated for a total of 15 minutes, followed by a 30 minute ‘rest’ period where lambs were supported via the intact placenta. A group of lambs was also euthanised at birth to act as an unventilated control group. At post-mortem lung tissue was sampled for proteomic analysis. Lung tissue samples were analysed by mass spectrometry-based proteomics using a TMT-11plex labelling approach, followed by nano-liquid chromatography coupled to an Q Exactive HF-X benchtop Orbitrap mass spectrometer in a data-dependent acquisition mode.
Project description:The associated files are mass spec data from two HPLC fractionations (Size Exclusion (SEC) or a triple phase ion exchange combination (WaxWaxCat)) of a single native protein extract from Selaginella moellendorfii. The extract was made from frozen powdered green tissue in 50 mM Tris pH 7.5, 150 mM NaCl, 5 mM EGTA, 10% glycerol, 1% NP40, phosphatase inhibitors and plant-specific protease inhibitors. 1 mg total protein was separated by SEC and 1.25 mg (diluted to 50 mM NaCl) by a concatenated series of three columns: PolyWaxLP, PolyWaxLP, and poly CAT A (PolyLC, Inc). The triple column series was eluted with a NaCl gradient. The ion exchange fractions # 55-60 were not processed for mass spectrometry becaue the A280 trace from the HPLC indicated insufficient protein present in those fractions.