The Gag Protein PEG10 Binds to RNA and Regulates Trophoblast Stem Cell Lineage Specification
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ABSTRACT: Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development (1). It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains (2). Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation (3). Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development.
INSTRUMENT(S): Orbitrap Fusion, LTQ Orbitrap Elite
ORGANISM(S): Mus Musculus (ncbitaxon:10090)
SUBMITTER: erik verschueren
PROVIDER: MSV000083229 | MassIVE | Fri Dec 14 10:54:00 GMT 2018
REPOSITORIES: MassIVE
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