Proteomics

Dataset Information

0

Human corneocyte cross-linked envelopes are comprised of a multitude of proteins

ABSTRACT: The cornified outer layer of epidermis (stratum corneum) is our major extra-pulmonary barrier to the environment. This layer results from a well-orchestrated program of terminal differentiation producing corneocytes filled primarily with keratin intermediate filaments tightly connected by disulfide bonding. This cytoskeleton is interconnected to an isopeptide cross-linked protein envelope at the cell periphery stabilized by transglutaminase cross-linking.The envelope serves as a scaffold for deposition of the lipid barrier containing w-OH-ceramides secreted from inside the maturing keratinocytes.That defects in the transglutaminase gene TGM1 are a major cause of autosomal recessive congenital ichthyosis testifies to the importance of proper envelope formation for barrier function.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Robert H. Rice  

PROVIDER: MSV000083265 | MassIVE | Wed Dec 19 17:42:00 GMT 2018

SECONDARY ACCESSION(S): PXD012122

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

Human stratum corneum proteomics reveals cross-linking of a broad spectrum of proteins in cornified envelopes.

Karim Noreen N   Phinney Brett S BS   Salemi Michelle M   Wu Pei-Wen PW   Naeem Muhammad M   Rice Robert H RH  

Experimental dermatology 20190501 5

Defects in keratinocyte transglutaminase (TGM1), resulting in an improper protein scaffold for deposition of the lipid barrier, comprise a major source of autosomal recessive congenital ichthyosis. For that reason, the composition and formation of the cornified (cross-linked) protein envelope of the epidermis have been of considerable interest. Since the isopeptide cross-linked protein components are not individually isolable once incorporated, purified envelopes were analysed by mass spectromet  ...[more]

Similar Datasets

Proteomics Proteomic Analysis of Loricrin Knockout Mouse Epidermis IBAQ
2016-12-14 | PXD003532 | Pride
Proteomics Proteomic Analysis of Loricrin Knockout Mouse Epidermis
2016-12-14 | PXD003576 | Pride
Transcriptomics Keratins control Nrf2 by regulating mitochondrial activity and barrier composition / Keratin-dependent TSLP expression suggests a link between skin blistering and atopic disease
2016-03-26 | E-GEOD-79590 | biostudies-arrayexpress
Transcriptomics Keratins control Nrf2 by regulating mitochondrial activity and barrier composition / Keratin-dependent TSLP expression suggests a link between skin blistering and atopic disease
2016-03-26 | GSE79590 | GEO
Proteomics The Amino-Terminal Part of Human Filaggrin-2 is a Component of Cornified Envelopes
2019-02-25 | PXD011833 | Pride
Transcriptomics Gene Expression Profiling in Pachyonychia Congenita Skin
2014-11-16 | E-GEOD-63326 | biostudies-arrayexpress
Transcriptomics Genome-wide analysis of gene expression in Krt1+/+ versus Krt1-/- epidermis and full-thickness skin.
2012-12-01 | E-GEOD-32951 | biostudies-arrayexpress
Transcriptomics Keratin-dependent TSLP expression suggests a link between skin blistering and atopic disease
2016-03-26 | E-GEOD-79596 | biostudies-arrayexpress
Metabolomics Fatty acid transport protein 4 is required for incorporation of saturated ultralong-chain fatty acids into epidermal ceramides and monoacylglycerols
2021-07-15 | MTBLS1138 | MetaboLights
Transcriptomics Gene Expression Profiling in Pachyonychia Congenita Skin
2014-11-16 | GSE63326 | GEO