The RHO GTPases Interaction Network: mapping GAPs, GEFs and effectors of classical and atypical RHO GTPases by proximity-dependent labeling (BioID)

ABSTRACT: BioID Controls for Flp-In T-REx HEK293 cells are the following; Empty Vector: VL_20151116_COT_01_HEK293_pcDNA5EV_HB VL_20151116_COT_02_HEK293_pcDNA5EV_HB VL_20151116_COT_03_HEK293_pcDNA5EV_HB VL_20151116_COT_04_HEK293_pcDNA5EV_HB BirA-Flag-EGFP: VL_20150825_COT_05_HB VL_20150825_COT_06_HB VL_20150825_COT_07_HB VL_20150825_COT_08_HB BirA-Flag-EGFP-CAAX: VL_20181019_COT_01_HB VL_20181019_COT_02_HB VL_20181019_COT_03_HB VL_20181019_COT_04_HB BioID samples for Flp-In T-REx HEK293 cells are the following; RAC1_WT: 2186_ZL_RAC1_WT_BR1_Velos1_P103_HB 2308_ZL_RAC1_WT_BR2_Velos1_HB RHOG_WT: VL_20170123_COT_01_HB VL_20170123_COT_02_HB RHOA_WT: VL_20160323_COT_04new2_HB VL_20180104_COT_01_HB CDC42_WT: VL_20180104_COT_03_HB VL_20180104_COT_04_HB RAC1_G15A: VL_20180105_COT_01_2_HB VL_20180105_COT_02_HB CDC42_G15A: VL_20180105_COT_03_HB VL_20180105_COT_04_HB RHOG_G15A: VL_20180108_COT_01_HB VL_20180108_COT_02_HB RHOA_G17A: VL_20180108_COT_03_HB VL_20180108_COT_04_HB CDC42_G12V: 2189_ZL_CDC42_G12V_BR1_Velos1_P103_HB 2263_ZL_CDC42_G12V_BR2_Velos1_HB RAC1_G12V: 1871_RAC1_G12V_BirA_Flag_HEK293_2-2_HB VL_20150825_COT_02_HB RAC2_G12V: 2199_ZL_RAC2_G12V_BR1_Velos1_P103_HB 2323_ZL_RAC2_G12V_BR2_Velos1_HB RAC3_G12V: 2200_ZL_RAC3_G12V_BR1_Velos1_P103_HB 2321_ZL_RAC3_G12V_BR2_Velos1_HB RHOA_G14V: 2190_ZL_RHOA_G14V_BR1_Velos1_P103_HB 2262_ZL_RHOA_G14V_BR2_Velos1_HB RHOB_G14V: 2191_ZL_RHOB_G14V_BR1_Velos1_P103_HB 2261_ZL_RHOB_G14V_BR2_Velos1_HB RHOBTB1_WT: 2201_ZL_RHOBTB1_WT_BR1_Velos1_P103_HB 2320_ZL_RHOBTB1_WT_BR2_Velos1_HB RHOBTB2_WT: 2202_ZL_RHOBTB2_WT_BR1_Velos1_P103_HB 2318_ZL_RHOBTB2_WT_BR2_Velos1_HB RHOC_G14V: 2192_ZL_RHOC_G14V_BR1_Velos1_P103_HB 2260_ZL_RHOC_G14V_BR2_Velos1_HB RHOD_G26V: 2193_ZL_RHOD_G26V_BR1_Velos1_P103_HB 2259_ZL_RHOD_G26V_BR2_Velos1_HB RHOF_G28V: 2246_ZL_RHOF_G28V_BR1_2194ReRun_Velos1_P103_HB 2258_ZL_RHOF_G28V_BR2_Velos1_HB RHOG_G12V: 2306_ZL_RHOG_G12V_BR2_Velos1_HB VL_20170123_COT_03_HB RHOH_WT: 2203_ZL_RHOH_WT_BR1_Velos1_P103_HB 2317_ZL_RHOH_WT_BR2_Velos1_HB RHOJ_G40V: 2195_ZL_RHOJ_G40V_BR1_Velos1_P103_HB 2257_ZL_RHOJ_G40V_BR2_Velos1_HB RHOQ_G18V: 2196_ZL_RHOQ_G18V_BR1_Velos1_P103_HB 2338_ZL_RHOQ_G18V_BR2_Velos1_HB RHOU_G58V: 2250_ZL_RHOU_G58v_BR1_2197ReRun_Velos1_P103_HB 2337_ZL_RHOU_G58V_BR2_Velos1_HB RHOV_G40V: 2198_ZL_RHOV_G40V_BR1_Velos1_P103_HB 2324_ZL_RHOV_G40V_BR2_Velos1_HB RND1_WT: 2249_ZL_RND1_WT_BR1_Velos1_P103_HB 2313_ZL_RND1_WT_BR2_Velos1_HB RND2_WT: 2247_ZL_RND2_WT_BR1_Velos1_P103_HB 2315_ZL_RND2_WT_BR2_Velos1_HB RND3_WT: VL_20151116_COT_05_HB VL_20151116_COT_06_HB BioID Controls for Flp-In T-REx HeLa cells are the following; Empty Vector: QE_20180917_COT_01_HB QE_20180917_COT_02_HB QE_20180917_COT_03_HB QE_20180917_COT_04_HB BirA-Flag-EGFP: QE_20151030_COT_02_HB QE_20151030_COT_03_HB QE_20151030_COT_04_HB QE_20151030_COT_05_HB BirA-Flag-EGFP-CAAX: QE_20181022_COT_01_HB QE_20181022_COT_02_HB QE_20181022_COT_03_HB QE_20181022_COT_04_HB BioID samples for Flp-In T-REx HeLa cells are the following; CDC42_G15A: QE_20171218_COT_05_HB QE_20171218_COT_06_HB CDC42_WT: QE_20171218_COT_01_HB QE_20171218_COT_02_HB RAC1_G15A: QE_20171218_COT_03_HB QE_20171218_COT_04_HB RAC1_WT: QE_20151102_COT_01_HB QE_20151102_COT_02_HB RHOA_G17A: QE_20171218_COT_07_HB QE_20171218_COT_08_HB RHOA_WT: QE_20171201_COT_07_HB QE_20171201_COT_08_HB RHOG_G15A: QE_20171220_COT_01_HB QE_20171220_COT_02_HB RHOG_WT: QE_20161215_COT_01_HB QE_20161215_COT_02_HB CDC42_G12V: QE_20171201_COT_09_HB QE_20171201_COT_10_HB RAC1_G12V: QE_20151030_COT_06_HB QE_20151030_COT_07_HB RAC2_G12V: QE_20180105_COT_05_HB QE_20180105_COT_06_HB RAC3_G12V: QE_20180105_COT_07_HB QE_20180105_COT_08_HB RHOA_G14V: QE_20171205_COT_17_HB QE_20171205_COT_18_HB RHOB_G14V: QE_20180105_COT_01_HB QE_20180105_COT_02_HB RHOBTB1_WT: QE_20180108_COT_07_HB QE_20180108_COT_08_HB RHOBTB2_WT: QE_20171205_COT_19_HB QE_20171205_COT_20_HB RHOC_G14V: QE_20180105_COT_03_HB QE_20180105_COT_04_HB RHOD_G26V: QE_20180108_COT_05_HB QE_20180108_COT_06_HB RHOF_G28V: QE_20171130_COT_05_HB QE_20171130_COT_06_HB RHOG_G12V: QE_20161215_COT_03_HB QE_20161215_COT_04_HB RHOH_WT: QE_20171204_COT_15_HB QE_20171204_COT_16_HB RHOJ_G40V: QE_20171130_COT_03_HB QE_20171130_COT_04_HB RHOQ_G18V: QE_20180105_COT_09_HB QE_20180105_COT_10_HB RHOU_G58V: QE_20171204_COT_11_HB QE_20171204_COT_12_new_HB RHOV_G40V: QE_20171130_COT_01_HB QE_20171130_COT_02_HB RND1_WT: QE_20180108_COT_01_HB QE_20180108_COT_02_HB RND2_WT: QE_20171204_COT_13_HB QE_20171204_COT_14_HB RND3_WT: QE_20180108_COT_03_HB QE_20180108_COT_04_HB

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jean Francois Cote  

PROVIDER: MSV000083608 | MassIVE | Thu Mar 21 09:59:00 GMT 2019

REPOSITORIES: MassIVE