Dataset Information

Paenibacillus larvae exoproteins of ERIC I-IV genotypes

ABSTRACT: The following strains of Paenibacillus larvae classified as ERIC I-IV genotypes (pl1-pl4) were used in the study: ERIC I - DSM-7030; ERIC II - DSM-25430; ERIC III - LMG-16252; and ERIC IV - DSM-3615. The 7 biological replicates (a-g) of the exoprotein fractions prepared from each ERIC genotype were used in the nanoLC-MS/MS analysis. All 28 samples were consecutively analyzed using an OrbitrapTM FusionTM Tribrid q-OT-IT mass spectrometer. To improve the protein identification and easy exclude contaminants from the medium, we considered the proteins that could originate from the yeast extract and Mueller-Hinton broth (contains beef fusion and casein peptone-acidic hydrolysate) in analyzing the MS data. Thus, we included the sequences related to Saccharomyces cerevisiae and Bos taurus, respectively, to the list of contaminants in MaxQuant. The contaminants.fasta file is provided. Futhermore, we provide the database (uniprot-paenibacillus+larvae.fasta) used for the identification of P. larvae proteins created of different strains of P. larvae. The sequences were downloaded of UniProt on 12.03.2019. Finally, the compressed (zipped) folder "combined" is provided. The data were analyzed in MaxQuant v1.6.3.4.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Paenibacillus Larvae (ncbitaxon:1464)

SUBMITTER: Tomas Erban

PROVIDER: MSV000083636 | MassIVE | Wed Mar 27 15:52:00 GMT 2019

SECONDARY ACCESSION(S): PXD013272

REPOSITORIES: MassIVE

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Publications

Comprehensive proteomic analysis of exoproteins expressed by ERIC I, II, III and IV Paenibacillus larvae genotypes reveals a wide range of virulence factors.

Erban Tomas T Zitek Justyna J Bodrinova Miroslava M Talacko Pavel P Bartos Milan M Hrabak Jaroslav J

Virulence 20191201 1

American foulbrood is a quarantine disease of the honeybee Apis mellifera L. in many countries and contributes greatly to colony losses. We performed a label-free proteomics study of exoprotein fractions produced in vitro by Paenibacillus larvae reference strains of the ERIC I-IV genotypes. A quantitative comparison was performed of previous studied protein-based virulence factors and many newly identified putative virulence factors. Among the multiple proteases identified, key virulence factors ...[more]

PMID: 30957692

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