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Proteomics analysis of HeLa cells starved with or without MG132

ABSTRACT: HeLa cells were amino acid starved for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin. The peptides were analyzed on a Thermofisher scientific Q Exactive HFX.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Bertrand Fabre

PROVIDER: MSV000083644 | MassIVE | Sat Mar 30 08:28:00 GMT 2019

REPOSITORIES: MassIVE

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Publications

Modulation of the cell cycle regulating transcription factor E2F1 pathway by the proteasome following amino acid starvation.

Fabre Bertrand B Livneh Ido I Ziv Tamar T Ciechanover Aaron A

Biochemical and biophysical research communications 20190413 3

The proteasome is one of the main catalytic machineries of eukaryotic cells responsible for protein degradation, and is known to be regulated during several cellular stress conditions. Recent studies suggest that the activity of the proteasome is modulated following mTOR inhibition. However, it is not clear how this process affects the proteome. In the present study, we investigated the role of the proteasome in the modulation of the proteome of HeLa cells following amino acid starvation, a stre ...[more]

PMID: 30992132

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