Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

FAC Sorted enrichment of mossy fiber synaptosomes

ABSTRACT: FAC Sorted mossy fiber synaptosomes. MS experiments to verify enrichment of cell type specific proteins

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Jeffrey N. Savas, PhD

PROVIDER: MSV000083683 | MassIVE | Sat Apr 13 17:07:00 BST 2019

SECONDARY ACCESSION(S): PXD013492

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression profile of FAC-sorted murine retinal cells

2011-07-12 | GSE29318 | GEO

Transcriptome of sorted ILC2s in mice fed high-fiber and control diet

Project description:To determine the activation patterns of ILC2s and associated ILC2-intrinsic functional molecules triggered by the high fiber diet, we administered either a control or high fiber diet in WT mice and performed RNA sequencing of FACS-sorted ILC2s from mouse colons. RNAseq libraries were prepared from 1,000 sorted colonic lamina propria ILC2s (CD45+Lin-CD90.2+CD127+KLRG1+) by the Epigenomics Core at WCM using the Clontech SMARTer® Ultra® Low Input RNA Kit V4 (Clontech Laboratories). Libraries were sequenced on an Illumina HiSeq 2500, generating 50 bp single-end reads. Two samples from two control diet-fed WT SPF mice and two samples from two high fiber diet-fed WT SPF mice were used.

2022-08-17 | GSE183443 | GEO

Expression profile of FAC-sorted murine retinal cells

Project description:Microarray experiments were performed using FAC-sorted young photoreceptors to analyze their transcriptome in comparison to remaining retinal cells at same developmental stage and retinal progenitors. For each replicate, retinae of 6 to 8 postnatal day 0 pNestin-GFP or postnatal day 4 rhoEGFP mice were dissected and FAC-sorted based on GFP expression. RNA of fractions was isolated and subsequently analyzed with microarray experiment.

2011-07-12 | E-GEOD-29318 | biostudies-arrayexpress

samon_1803_DIA_HSPC - Sensitive quantitative proteomics of human hematopoietic stem and progenitor cells by data-independent acquisition mass spectrometry

Project description:- tryptic digests of CD34+ FAC-sorted cells dilution series acquired in DIA mode on Orbitrap Lumos - tryptic digests of HSC, MEP, GMP, CMP FAC-sorted cells acquired in DIA mode on Orbitrap Lumos

2019-04-25 | PXD009255 | Pride

Castor fiber fiber

Project description:Castor fiber fiber Raw sequence reads

| PRJNA313146 | ENA

RNA-seq of FAC sorted leaf palisade cells

Project description:RNA-seq of FAC sorted leaf palisade cells

| PRJNA756203 | ENA

Transcriptome of sorted ILC2s in mice fed high-fiber and control diet

Project description:Transcriptome of sorted ILC2s in mice fed high-fiber and control diet

| PRJNA760885 | ENA

RNA Profiling of FAC-Sorted Neurons From the Developing Zebrafish Spinal Cord.

Project description:In this report, we describe a successful protocol for isolating and expression-profiling live fluorescent- protein-labelled neurons from zebrafish embryos. As a proof-of-principle for this method, we FAC-sorted and RNA-profiled GFP-labelled spinal CiA interneurons and compared the expression profile of these cells to those of post-mitotic spinal neurons in general and to all trunk cells. We show that RNA of sufficient quality and quantity to uncover both expected and novel transcription profiles via Affymetrix microarray analysis can be extracted from 5,700 to 20,000 FAC-sorted cells. As part of this study, we also further confirm the genetic homology of mammalian and zebrafish V1 interneurons, by demonstrating that zebrafish V1 cells (CiAs) express genes that encode for the transcription factors Lhx1a and Lhx5. This protocol for dissociating, sorting and RNA-profiling neurons from organogenesis-stage zebrafish embryos should also be applicable to other developing organs and tissues and potentially other model organisms.

2020-02-26 | GSE145916 | GEO

Castor fiber

Project description:Castor fiber Assembly

| PRJNA355853 | ENA

Castor fiber

Project description:Castor fiber overview

| PRJNA184180 | ENA