Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of FAC sorted leaf palisade cells

ABSTRACT: RNA-seq of FAC sorted leaf palisade cells

PROVIDER: PRJNA756203 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15526000.fastq.gz	Fastqsanger.gz
	SRR15526001.fastq.gz	Fastqsanger.gz
	SRR15526002.fastq.gz	Fastqsanger.gz
	SRR15526003.fastq.gz	Fastqsanger.gz
	SRR15526004.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 27

Similar Datasets

RNA-seq of FAC sorted leaf palisade cells

Project description:Arabidopsis leaf protoplasts were sorted to generate palisade cell and non-palisade photosynthetic reference cell populations. Treatments include with and without transcription inhibitors, and high light.

2022-08-11 | GSE182414 | GEO

Expression profile of FAC-sorted murine retinal cells

Project description:Microarray experiments were performed using FAC-sorted young photoreceptors to analyze their transcriptome in comparison to remaining retinal cells at same developmental stage and retinal progenitors. For each replicate, retinae of 6 to 8 postnatal day 0 pNestin-GFP or postnatal day 4 rhoEGFP mice were dissected and FAC-sorted based on GFP expression. RNA of fractions was isolated and subsequently analyzed with microarray experiment.

2011-07-12 | E-GEOD-29318 | biostudies-arrayexpress

Expression profile of FAC-sorted murine retinal cells

2011-07-12 | GSE29318 | GEO

RNA Profiling of FAC-Sorted Neurons From the Developing Zebrafish Spinal Cord.

Project description:In this report, we describe a successful protocol for isolating and expression-profiling live fluorescent- protein-labelled neurons from zebrafish embryos. As a proof-of-principle for this method, we FAC-sorted and RNA-profiled GFP-labelled spinal CiA interneurons and compared the expression profile of these cells to those of post-mitotic spinal neurons in general and to all trunk cells. We show that RNA of sufficient quality and quantity to uncover both expected and novel transcription profiles via Affymetrix microarray analysis can be extracted from 5,700 to 20,000 FAC-sorted cells. As part of this study, we also further confirm the genetic homology of mammalian and zebrafish V1 interneurons, by demonstrating that zebrafish V1 cells (CiAs) express genes that encode for the transcription factors Lhx1a and Lhx5. This protocol for dissociating, sorting and RNA-profiling neurons from organogenesis-stage zebrafish embryos should also be applicable to other developing organs and tissues and potentially other model organisms.

2020-02-26 | GSE145916 | GEO

FAC Sorted enrichment of mossy fiber synaptosomes

Project description:FAC Sorted mossy fiber synaptosomes. MS experiments to verify enrichment of cell type specific proteins

2019-04-13 | MSV000083683 | MassIVE

RNA Profiling of FAC-Sorted Neurons From the Developing Zebrafish Spinal Cord.

Project description:RNA Profiling of FAC-Sorted Neurons From the Developing Zebrafish Spinal Cord.

| PRJNA608730 | ENA

samon_1803_DIA_HSPC - Sensitive quantitative proteomics of human hematopoietic stem and progenitor cells by data-independent acquisition mass spectrometry

Project description:- tryptic digests of CD34+ FAC-sorted cells dilution series acquired in DIA mode on Orbitrap Lumos - tryptic digests of HSC, MEP, GMP, CMP FAC-sorted cells acquired in DIA mode on Orbitrap Lumos

2019-04-25 | PXD009255 | Pride

Francisella tularensis strain:FAC

Project description:Francisella tularensis strain:FAC Genome sequencing and assembly

| PRJNA244553 | ENA

Array tomography: characterizing FAC-sorted populations of zebrafish immune cells by their 3D ultrastructure.

Project description:For 3D reconstructions of whole immune cells from zebrafish, isolated from adult animals by FAC-sorting we employed array tomography on hundreds of serial sections deposited on silicon wafers. Image stacks were either recorded manually or automatically with the newly released ZEISS Atlas 5 Array Tomography platform on a Zeiss FEGSEM. To characterize different populations of immune cells, organelle inventories were created by segmenting individual cells. In addition, arrays were used for quantification of cell populations with respect to the various cell types they contained. The detection of immunological synapses in cocultures of cell populations from thymus or WKM with cancer cells helped to identify the cytotoxic nature of these cells. Our results demonstrate the practicality and benefit of AT for high-throughput ultrastructural imaging of substantial volumes.

| S-EPMC4670706 | biostudies-literature

RNA-seq of sorted pancreatic endocrine progenitor cells

Project description:RNA-seq of sorted pancreatic endocrine progenitor cells

| PRJNA630435 | ENA