Dataset Information

Activity-based protein profiling of xylan-degrading glycoside hydrolases in Aspergillus secretomes

ABSTRACT: Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides towards breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling (ABPP) provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection and identification of b-xylosidases and endo-xylanases in the secretome of Aspergillus niger, by the use of chemical probes inspired by the b-glucosidase inhibitor cyclophellitol. Furthermore, we demonstrate the use of these activity-based probes (ABPs) to assess enzyme-substrate specificities, thermal stabilities, and other biotechnologically relevant parameters. Our experiments highlight the utility of ABPs as promising tools for the discovery of relevant enzymes useful for biomass breakdown.

INSTRUMENT(S): maXis

ORGANISM(S): Aspergillus Niger (ncbitaxon:5061)

SUBMITTER: Gideon J. Davies

PROVIDER: MSV000083714 | MassIVE | Wed Apr 24 04:09:00 BST 2019

SECONDARY ACCESSION(S): PXD013618

REPOSITORIES: MassIVE

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Publications

Dynamic and Functional Profiling of Xylan-Degrading Enzymes in <i>Aspergillus</i> Secretomes Using Activity-Based Probes.

Schröder Sybrin P SP de Boer Casper C McGregor Nicholas G S NGS Rowland Rhianna J RJ Moroz Olga O Blagova Elena E Reijngoud Jos J Arentshorst Mark M Osborn David D Morant Marc D MD Abbate Eric E Stringer Mary A MA Krogh Kristian B R M KBRM Raich Lluís L Rovira Carme C Berrin Jean-Guy JG van Wezel Gilles P GP Ram Arthur F J AFJ Florea Bogdan I BI van der Marel Gijsbert A GA Codée Jeroen D C JDC Wilson Keith S KS Wu Liang L Davies Gideon J GJ Overkleeft Herman S HS

ACS central science 20190524 6

Plant polysaccharides represent a virtually unlimited feedstock for the generation of biofuels and other commodities. However, the extraordinary recalcitrance of plant polysaccharides toward breakdown necessitates a continued search for enzymes that degrade these materials efficiently under defined conditions. Activity-based protein profiling provides a route for the functional discovery of such enzymes in complex mixtures and under industrially relevant conditions. Here, we show the detection a ...[more]

PMID: 31263766

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