Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Ovis Aries (ncbitaxon:9940)
SUBMITTER: Arlindo Moura
PROVIDER: MSV000083725 | MassIVE | Thu Apr 25 11:55:00 BST 2019
SECONDARY ACCESSION(S): PXD013638
REPOSITORIES: MassIVE
Sanchez Deisy J D DJD Vasconcelos Fabio R FR Teles-Filho Antônio C A ACA Viana Arabela G A AGA Martins Aline M A AMA Sousa Marcelo V MV Castro Mariana S MS Ricart Carlos A CA Fontes Wagner W Bertolini Marcelo M Bustamante-Filho Ivan C IC Moura Arlindo A AA
Reproduction in domestic animals = Zuchthygiene 20210202 4
The present study was conducted to decipher the proteome of in vivo-produced pre-implantation ovine embryos. Ten locally adapted Morana Nova ewes received hormonal treatment and were inseminated 12 hr after ovulation. Six days later, 54 embryos (morula and blastocyst developmental state) were recovered from eight ewes and pooled to obtain sufficient protein for proteomic analysis. Extracted embryo proteins were analysed by LC-MS/MS, followed by identification based on four database searches (PEA ...[more]