Proteomics

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Increased Hemodynamic Load in Early Embryonic Stages Alters Endocardial to Mesenchymal Transition

ABSTRACT: Normal blood flow is essential for proper heart formation during embryonic development, as abnormal hemodynamic load (blood pressure and shear stress) results in cardiac defects seen in congenital heart disease. However, the progressive detrimental remodeling processes that relate altered blood flow to cardiac defects remain unclear. Outflow tract banding was used to increase hemodynamic load in the chicken embryo heart between Hamburger and Hamilton stages 18 and 24. Increased hemodynamic load induced increased cell density in outflow tract cushions, fewer cells along the endocardial lining, endocardium junction disruption, and altered periostin expression as measured by confocal microscopy analysis. Proteomic mass-spectrometry analysis quantified altered protein composition after banding.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Gallus Gallus (ncbitaxon:9031)

SUBMITTER: Sandra Rugonyi  

PROVIDER: MSV000084103 | MassIVE | Wed Jul 17 17:18:00 BST 2019

SECONDARY ACCESSION(S): PXD005362

REPOSITORIES: MassIVE

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Increased Hemodynamic Load in Early Embryonic Stages Alters Myofibril and Mitochondrial Organization in the Myocardium.

Midgett Madeline M   López Claudia S CS   David Larry L   Maloyan Alina A   Rugonyi Sandra S  

Frontiers in physiology 20170830

Normal blood flow is essential for proper heart formation during embryonic development, as abnormal hemodynamic load (blood pressure and shear stress) results in cardiac defects seen in congenital heart disease (CHD). However, the detrimental remodeling processes that relate altered blood flow to cardiac malformation and defects remain unclear. Heart development is a finely orchestrated process with rapid transformations that occur at the tissue, cell, and subcellular levels. Myocardial cells pl  ...[more]

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