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Regional differences in left ventricular shear stress contribute to heterogenity of the endocardial transcriptome

ABSTRACT: The role of shear stress, the frictional force of blood flow, on the endothelium has been well documented. Differences in shear stress can have profound effects on endothelial and blood vessel biology. Endothelial cells (ECs), termed endocardial ECs, line the heart chambers and are exposed to complex shear stress patterns. While it has been demonstrated that shear stress is important for heart development, little has been shown on the role of shear stress on adult ECs. 4D-MRI studies demonstrate regional differences in blood residence time. We sought to determine the effect of regional differences in endocardial shear stress on the endocardial transcriptome using RNA sequencing (RNA-seq) on 3 different regions (apex, mid-ventricle, outflow tract) from 8 adult pigs, for a total of 24 RNA-seq assays.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Sus scrofa

SUBMITTER: Peter Davies

PROVIDER: E-MTAB-3669 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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