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Comprehensive spectral library from the pathogenic bacterium Streptococcus pneumoniae with focus on phosphoproteins

ABSTRACT: In this study, a comprehensive spectral library for S. pneumoniae with an emphasis on phosphopeptide spectra was compiled. In total, 76% of the theoretical S. pneumoniae proteins were stored in a spectral library. Additionally, in combination with classical database search algorithms and the spectral library search, identification of 128 phosphoproteins was feasible. The phosphopeptides in the spectral library were manually validated using synthetic phosphopeptides to improve the quality of the library spectra and to minimize false positive spectra.

INSTRUMENT(S): LTQ Orbitrap, LTQ Velos

ORGANISM(S): Streptococcus Pneumoniae D39

SUBMITTER: Doerte Becher

PROVIDER: MSV000084269 | MassIVE |

SECONDARY ACCESSION(S): PXD015291

REPOSITORIES: MassIVE

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Comprehensive Spectral Library from the Pathogenic Bacterium <i>Streptococcus pneumoniae</i> with Focus on Phosphoproteins.

Hentschker Christian C Maaß Sandra S Junker Sabryna S Hecker Michael M Hammerschmidt Sven S Otto Andreas A Becher Dörte D

Journal of proteome research 20200323 4

To understand bacterial reactions to environmental stress or infection-related processes, it is necessary to identify the involved proteins. In mass spectrometry-based proteomics, the method of choice for spectra-to-peptide-match is database search, but in recent times, spectral libraries have come into focus. Here, we built a mass spectral library from <i>Streptococcus pneumoniae</i> D39, reflecting 76% of the theoretical proteome of the organism. Besides the proteins themselves, posttranslatio ...[more]

PMID: 32154730

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