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Simulated spectral library searching on TripleTOF phosphopeptide datasets

ABSTRACT: Synthetic phosphopeptides and enriched K562 cell phosphopeptides were analyzed using a TripleTOF 6600 mass spectrometer before and after enzymatic dephosphorylation. Dephosphorylated peptides identified by X!Tandem database searching were subjected to spectral simulation of all possible single phosphorylations using SimPhospho software. Phosphopeptides were identified and localized by SpectraST searching against a library of the simulated spectra, and also by MaxQuant database searching.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Susumu Y. Imanishi

PROVIDER: PXD012433 | JPOST Repository | Tue Dec 03 00:00:00 GMT 2019

REPOSITORIES: jPOST

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			Action	DRS
	20160901_04_Blue_dephospho.wiff	Wiff
	20160901_04_Blue_dephospho.wiff.scan	Wiff
	20160901_05_Blue_dephospho.wiff	Wiff
	20160901_05_Blue_dephospho.wiff.scan	Wiff
	20160901_06_Blue_dephospho.wiff	Wiff

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Publications

Optimization of TripleTOF spectral simulation and library searching for confident localization of phosphorylation sites.

Takai Ayano A Tsubosaka Tomoya T Hirano Yasuhiro Y Hayakawa Naoki N Tani Fumitaka F Haapaniemi Pekka P Suni Veronika V Imanishi Susumu Y SY

PloS one 20191202 12

Tandem mass spectrometry (MS/MS) has been used in analysis of proteins and their post-translational modifications. A recently developed data analysis method, which simulates MS/MS spectra of phosphopeptides and performs spectral library searching using SpectraST, facilitates confident localization of phosphorylation sites. However, its performance has been evaluated only on MS/MS spectra acquired using Orbitrap HCD mass spectrometers so far. In this study, we have investigated whether this appro ...[more]

PMID: 31790495

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