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The anti-MRSA compound KCR inhibits protein synthesis in Staphylococcus aureus

ABSTRACT: S aureus was treated in triplicate with 3-O-alpha-L-(2",3"-di-p-coumaroyl)rhamnoside (KCR), oxacillin or vehicle and quantitative proteomic analysis was carried out using isobaric tags and mass spectrometry. 1190 proteins were identified and 552 were affected by KCR (q<0.01). Ontology analysis identified 6 distinct translation-related categories that were affected by KCR (PIANO, 10% false-discovery rate) including structural constituent of ribosome, translation, rRNA binding, tRNA binding, tRNA processing and aminoacyl-tRNA ligase activity. Median fold changes (KCR vs Control) for small and large ribosomal components were 1.46 and 1.43 respectively.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus (ncbitaxon:1280)

SUBMITTER: Nicholas Carruthers

PROVIDER: MSV000084282 | MassIVE |

SECONDARY ACCESSION(S): PXD015333

REPOSITORIES: MassIVE

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The anti-MRSA compound 3-O-alpha-L-(2″,3″-di-p-coumaroyl)rhamnoside (KCR) inhibits protein synthesis in Staphylococcus aureus.

Carruthers Nicholas J NJ Stemmer Paul M PM Media Joe J Swartz Ken K Wang Xiaojuan X Aube Nicholas N Hamann Mark T MT Valeriote Frederick F Shaw Jiajiu J

Journal of proteomics 20191017

Methicillin-resistant S aureus (MRSA) contributes to patient mortality and extended hospital stays. 3-O-alpha-L-(2″,3″-di-p-coumaroyl)rhamnoside (KCR) is a natural product antibiotic that is effective against MRSA but has no known mechanism of action (MOA). We used proteomics to identify the MOA for KCR. Methicillin sensitive S aureus and a mixture of four KCR stereoisomers were tested. A time-kill assay was used to choose a 4 h treatment using KCR at 5× its MIC for proteomic analysis. S aureus ...[more]

PMID: 31629958

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