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Exoprotoemics of pseudomonas putida KT2440 outer membrane vesicles (OMV) and vesicle-free secretome (VSF)

ABSTRACT: Lignin is an abundant and recalcitrant component of plant cell walls. While lignin degradation in Nature is typically attributed to fungi, growing evidence suggests that bacteria can also modify this complex biopolymer. However, the spatiotemporal mechanisms for this modification remain unclear. Improved understanding of this biological and extracellular process would aid in our collective knowledge of both carbon cycling and advanced microbial strategies to valorize lignin. In this data set, we examined the exoproteome of Pseudomonas putida KT2440 grown with on lignin-rich media to characterize the secretion of abundant outer membrane vesicles (OMVs). Proteomic analysis revealed that the exoproteome is 20% more sub-compartmentalized into OMVs and OMV protein cargo are 52% more dynamic in lignin-rich media as compared to lignin-free media.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Pseudomonas Putida Kt2440 (ncbitaxon:160488)

SUBMITTER: Gregg Beckham

PROVIDER: MSV000084506 | MassIVE | Fri Oct 25 12:38:00 BST 2019

SECONDARY ACCESSION(S): PXD016028

REPOSITORIES: MassIVE

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