Proteomics

Dataset Information

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MHtt tobacco roots proteome, QEHFX


ABSTRACT: Samples were analyzed using a nanoACQUITY UPLC system (Waters) coupled to a Q-Exactive HF-X high resolution accurate mass tandem mass spectrometer (Thermo) via a nanoelectrospray ionization source. Briefly the sample was first trapped on a Symmetry C18 180 micron x 20 mm trapping column (5 microliters/min at 99.9/0.1 v/v H2O/MeCN) followed by an analytical separation using a 1.7 micron AQCUITY HSS T3 C18 75 micron x 250 mm column (Waters) with a 90 min gradient of 5 to 30% MeCN with 0.1% formic acid at a flow rate of 400 nl/min and column temperature of 55 degC. Data collection on the HF-X MS was performed in data-dependent acquisition (DDA) mode with a 120,000 resolution (at m/z 200) full MS scan from m/z 375 to 1600 with a target AGC value of 3e6 ions and 50 ms maximum injection time (IT). The Top30 peptides were selected for MS/MS using a resolution of 15,000, max AGC of 5e4 ions and minimum AGC target of 2.25e3, max IT of 45 ms, collision energy of 27, an isolation width of 1.2 m/z and 20 s dynamic exclusion.

INSTRUMENT(S): Q-Exactive HF-X

ORGANISM(S): Nicotiana Tabacum (ncbitaxon:4097)

SUBMITTER: JiaHua Xie  

PROVIDER: MSV000084557 | MassIVE | Thu Nov 07 21:34:00 GMT 2019

REPOSITORIES: MassIVE

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Publications

A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR.

Hung Chiu-Yueh CY   Zhu Chuanshu C   Kittur Farooqahmed S FS   He Maotao M   Arning Erland E   Zhang Jianhui J   Johnson Asia J AJ   Jawa Gurpreet S GS   Thomas Michelle D MD   Ding Tomas T TT   Xie Jiahua J  

Cellular and molecular life sciences : CMLS 20221017 11


Pathophysiology associated with Huntington's disease (HD) has been studied extensively in various cell and animal models since the 1993 discovery of the mutant huntingtin (mHtt) with abnormally expanded polyglutamine (polyQ) tracts as the causative factor. However, the sequence of early pathophysiological events leading to HD still remains elusive. To gain new insights into the early polyQ-induced pathogenic events, we expressed Htt exon1 (Htt<sub>ex1</sub>) with a normal (21), or an extended (4  ...[more]

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