Project description:SCAP-XL of Halo-WDR76 and SNAP-SPIN1 from stable cell line in WDR76 KO background. Serial Capture Affinity Purification (SCAP) was performed on whole cell extract of a stable cell line of 293FRT cells in WDR76 KO background. DSSO crosslinked in E2 sample. Data was acquired using a ms1/ms2/ms3 method. Data was searched using Proteomics Discoverer 2.4 with XlinkX.
Project description:Halo purified WDR76 from a stable cell line of 293FRT in WDR76 KO background. DSSO crosslinking performed in eluted samples. Data collected using ms1/ms2/ms3 method. Data searched using Proteomics Discoverer 2.4 with XlinkX.
Project description:Serial Capture Affinity Purification (SCAP) samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are SNAP-SPIN1 and Halo-WDR76. SNAP purification was performed first, then Halo purification was performed. SCAP was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. 3 biological replicates of E1, UB2 and E2 fractions.
Project description:HeLa/16E6-16E7 cells after expression of wild-type E2 compared to an E2 DNA-binding mutant Keywords: Wild-type E2 Infection vs. Mutant E2 Infection