Proteomics

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WDR76-SPIN1_inWDR76KO_SCAP-MS_MudPIT

ABSTRACT: Serial Capture Affinity Purification (SCAP) samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are SNAP-SPIN1 and Halo-WDR76. SNAP purification was performed first, then Halo purification was performed. SCAP was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. 3 biological replicates of E1, UB2 and E2 fractions.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael P. Washburn  

PROVIDER: MSV000086802 | MassIVE | Wed Feb 03 14:45:00 GMT 2021

REPOSITORIES: MassIVE

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