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Alterations in Bacillus subtilis caused by high salinity and the compatible solute glycine betaine

ABSTRACT: Cells were grown aerobically at 37 degC in Spizizen minimal medium either supplemented with 1 mM glycine betaine, 1.2 M NaCl or 1.2 M NaCl and 1 mM glycine betaine. Cells were harvested in the exponential growth phase at an OD600nm of 1.0. Proteome analysis was performed with samples from three biological replicates.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423)

SUBMITTER: Prof. Uwe Voelker

PROVIDER: MSV000084766 | MassIVE | Wed Jan 08 04:31:00 GMT 2020

REPOSITORIES: MassIVE

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Impact of high salinity and the compatible solute glycine betaine on gene expression of Bacillus subtilis.

Rath Hermann H Sappa Praveen K PK Hoffmann Tamara T Gesell Salazar Manuela M Reder Alexander A Steil Leif L Hecker Michael M Bremer Erhard E Mäder Ulrike U Völker Uwe U

Environmental microbiology 20200608 8

The Gram-positive bacterium Bacillus subtilis is frequently exposed to hyperosmotic conditions. In addition to the induction of genes involved in the accumulation of compatible solutes, high salinity exerts widespread effects on B. subtilis physiology, including changes in cell wall metabolism, induction of an iron limitation response, reduced motility and suppression of sporulation. We performed a combined whole-transcriptome and proteome analysis of B. subtilis 168 cells continuously cultivate ...[more]

PMID: 32419322

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Impact of high salinity and the compatible solute glycine betaine on global gene expression of Bacillus subtilis

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