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IP-MS identification of protein interaction for Na (+), K (+)-ATPase beta1 subunit

ABSTRACT: This project aims to identify proteins that may interact with the sodium potassium pump (ATPase) beta1 subunit in the human lung.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: David A. Dean

PROVIDER: MSV000084881 | MassIVE | Sun Feb 02 13:56:00 GMT 2020

REPOSITORIES: MassIVE

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Publications

The Na+, K+-ATPase β1 subunit regulates epithelial tight junctions via MRCKα.

Bai Haiqing H Zhou Rui R Barravecchia Michael M Norman Rosemary R Friedman Alan A Yu Deborah D Lin Xin X Young Jennifer L JL Dean David A DA

JCI insight 20210222 4

An intact lung epithelial barrier is essential for lung homeostasis. The Na+, K+-ATPase (NKA), primarily serving as an ion transporter, also regulates epithelial barrier function via modulation of tight junctions. However, the underlying mechanism is not well understood. Here, we show that overexpression of the NKA β1 subunit upregulates the expression of tight junction proteins, leading to increased alveolar epithelial barrier function by an ion transport-independent mechanism. Using IP and mas ...[more]

PMID: 33507884

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