Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Title: XL-MS analysis of protein isolates from Flp-In 293 cells stably expressing SIN3B isoform 2-HaloTag using DSSO crosslinker.

ABSTRACT: XL-MS analysis of protein isolates from Flp-In 293 cells stably expressing SIN3B isoform 2-HaloTag using DSSO crosslinker.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael P. Washburn

PROVIDER: MSV000085084 | MassIVE | Wed Mar 11 11:38:00 GMT 2020

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

XL-MS analysis of protein isolates from Flp-In 293 cells stably expressing SIN3A-HaloTag using DSSO crosslinker.

Project description:XL-MS analysis of protein isolates from Flp-In 293 cells stably expressing SIN3A-HaloTag using DSSO crosslinker.

2020-03-11 | MSV000085083 | MassIVE

HaloTag-based conjugation of proteins to barcoding-oligonucleotides

Project description:We describe the development of a high-sensitivity protein quantification system called HaloTag protein barcoding assay. The assay involves target protein linking to a unique molecule-counting oligonucleotide by click chemistry.

2019-11-04 | GSE122542 | GEO

AP-MS analysis using Flp-In-293 cells stably expressing SIN3B isoform 1 with C-terminal HaloTag

Project description:AP-MS analysis using Flp-In-293 cells stably expressing SIN3B isoform 1 with C-terminal HaloTag

2020-03-30 | MSV000085194 | MassIVE

AP-MS analysis using Flp-In-293 cells stably expressing SIN3B isoform 2 with C-terminal HaloTag

Project description:AP-MS analysis using Flp-In-293 cells stably expressing SIN3B isoform 2 with C-terminal HaloTag

2020-03-30 | MSV000085197 | MassIVE

AP-MS analysis using Flp-In-293 cells stably expressing SIN3B isoform 3 with C-terminal HaloTag

Project description:AP-MS analysis using Flp-In-293 cells stably expressing SIN3B isoform 3 with C-terminal HaloTag

2020-03-30 | MSV000085195 | MassIVE

p65-HaloTag copurifying proteins in human 293T cells

Project description:In effort to develop methodology for targeted top down mass spectrometry of NF kappa B p65 from human cells, we evaluated the utility of HaloTag for purification and analysis of recombinant protein. During our study, two datasets of bottom up LC-MS/MS were generated: one from in-gel digestion of the predominant band following p65-HaloTag purification, another from in-solution digestion of all the proteins present in a p65-HaloTag purification. p65-HaloTag copurifying proteins identified in our datasets include the known interactors c-Rel, NF-kappaB p105, NF-kappaB p100, and NF-kappaB inhibitor beta. Over 100 proteins were identified by at least two peptides using a Mascot ion cut-off score of 30.

2018-10-19 | PXD001932 | Pride

AP-MS analysis using Flp-In 293 cells stably expressing MORF4L1-HaloTag

Project description:AP-MS analysis using Flp-In 293 cells stably expressing MORF4L1-HaloTag

2020-03-10 | MSV000085076 | MassIVE

DSSO Crosslinking of Intact Tetrahymena Thermophila Cilia

Project description:The MS-cleavable crosslinker, DSSO, was added to cilia isolated from Tetrahymena thermophila. After digestion crosslinked peptides were enriched by size exclusion chromatography. Data were collected using an MS2/MS3 method. Analysis was done using the XlinkX node of PD2.3 and a fasta file generated from standard MS1/MS2 analysis of the crosslinked samples.

2022-07-19 | MSV000089917 | MassIVE