Decoding the protein composition of whole nucleosomes with Nuc-MS
Ontology highlight
ABSTRACT: Nuc-MS characterizes histone modifications and variants directly from intact
endogenous mono-nucleosomes. Preserving whole nucleosome particles enables
precise interrogation of their protein content, as for H3.3-containing nucleosomes which
had 6-fold co-enrichment of variant H2A.Z over bulk chromatin. Moreover, Nuc-MS
showed co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., H4K16ac
and >40-fold enrichment of H3K79me2). By capturing the entire epigenetic landscape,
Nuc-MS provides a new, quantitative readout of nucleosome-level biology.
INSTRUMENT(S): Q Exactive HF Ultra High Mass Range (Thermo Scientific)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: Neil L Kelleher
PROVIDER: MSV000085238 | MassIVE | Mon Apr 06 13:26:00 BST 2020
REPOSITORIES: MassIVE
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