Proteomics

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Decoding the protein composition of whole nucleosomes with Nuc-MS


ABSTRACT: Nuc-MS characterizes histone modifications and variants directly from intact endogenous mono-nucleosomes. Preserving whole nucleosome particles enables precise interrogation of their protein content, as for H3.3-containing nucleosomes which had 6-fold co-enrichment of variant H2A.Z over bulk chromatin. Moreover, Nuc-MS showed co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., H4K16ac and >40-fold enrichment of H3K79me2). By capturing the entire epigenetic landscape, Nuc-MS provides a new, quantitative readout of nucleosome-level biology.

INSTRUMENT(S): Q Exactive HF Ultra High Mass Range (Thermo Scientific)

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Neil L Kelleher  

PROVIDER: MSV000085238 | MassIVE | Mon Apr 06 13:26:00 BST 2020

REPOSITORIES: MassIVE

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