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Mitovesicles are mitochondria derived extracellular vesicles altered by aging and disease

ABSTRACT: Mitochondrial dysfunction is an established hallmark of aging and neural degenerative disorders such as Down syndrome and Alzheimer disease. Employing a high resolution density gradient on extracellular vesicles isolated from murine and human Down syndrome and diploid control brains, we identify and characterize a formerly unknown population of extracellular vesicles containing multiple mitochondrial markers that are distinct from the previously described EVs subtypes, microvesicles and exosomes. We term these mitochondria-derived EVs mitovesicles. We show that brain mitovesicle levels and cargo are tightly regulated under normal conditions and are altered during pathophysiological processes in which mitochondrial dysfunction occurs, suggesting that mitovesicles are a previously unrecognized player in mitochondria quality control and may have an active role in the intercellular tissue response to oxidative stress.

INSTRUMENT(S): Thermo Electron Q Exactive High Field Mass Spectrometer

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Thomas A. Neubert

PROVIDER: MSV000085447 | MassIVE | Tue May 19 10:36:00 BST 2020

REPOSITORIES: MassIVE

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