Proteomics

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Data, reagents, assays and merits of proteomics for SARS-CoV-2 research and testing

ABSTRACT: As the SARS-CoV-2 pandemic continues to spread, thousands of scientists around the globe have changed research direction to understand better how the virus works and to find out how it may be tackled. To facilitate proteomic research on SARS-CoV-2, we presents deep-scale proteomes of common cell line models (Calu-3, Caco-2, ACE2 transfected A549 and Vero E6) and monitors changes of the proteome upon viral infection in Vero E6 cells. We provide high quality proteome expression data that can be used to refine the annotation of protein coding regions of the Vero E6 cell line genome. Further, we generated spectral libraries that can be used DIA cell line experiments or PRM assays of SARS-CoV-2 proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Chlorocebus Sabaeus (ncbitaxon:60711) Chlorocebus Aethiops (ncbitaxon:9534) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Bernhard Kuster  

PROVIDER: MSV000085774 | MassIVE | Fri Jul 17 14:15:00 BST 2020

SECONDARY ACCESSION(S): PXD019645

REPOSITORIES: MassIVE

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Data, Reagents, Assays and Merits of Proteomics for SARS-CoV-2 Research and Testing.

Zecha Jana J   Lee Chien-Yun CY   Bayer Florian P FP   Meng Chen C   Grass Vincent V   Zerweck Johannes J   Schnatbaum Karsten K   Michler Thomas T   Pichlmair Andreas A   Ludwig Christina C   Kuster Bernhard B  

Molecular & cellular proteomics : MCP 20200626 9

As the COVID-19 pandemic continues to spread, thousands of scientists around the globe have changed research direction to understand better how the virus works and to find out how it may be tackled. The number of manuscripts on preprint servers is soaring and peer-reviewed publications using MS-based proteomics are beginning to emerge. To facilitate proteomic research on SARS-CoV-2, the virus that causes COVID-19, this report presents deep-scale proteomes (10,000 proteins; >130,000 peptides) of  ...[more]

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