Dataset Information

Blood to plasma processing : time-anticoagulant

ABSTRACT: This dataset contains raw mass spectrometry data (in the form of Thermo Fisher .RAW files) supporting associated publication “Variable blood processing procedures contribute to plasma proteomic variability” by Halvey et al. The experiments characterized by these data evaluated effects on human plasma proteome of processing delay (from blood draw to the first centrifugation: 0, 6, 24, 48, 72 and 144 hours) and the type of anticoagulant tube used for blood collection (heparin or EDTA). Sample names indicate combinations of corresponding conditions - e.g. 48h_hep = 48 hours in heparin tube. For the “6h_EDTA” experiment there were several instances of LC-MS/MS analytical replicates for this sample. It should be noted that for this experiment the .RAW file from the “./updates/2020-08-03_vfarutin_60de013e/raw/” folder under this dataset FTP address represents the actual LC-MS/MS run used to generate the data in the associated paper by Halvey et al.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anthony M. Manning

PROVIDER: MSV000085799 | MassIVE | Tue Jul 21 08:28:00 BST 2020

REPOSITORIES: MassIVE

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Variable blood processing procedures contribute to plasma proteomic variability.

Halvey Patrick P Farutin Victor V Koppes Laura L Gunay Nur Sibel NS Pappas Dimitrios A DA Manning Anthony M AM Capila Ishan I

Clinical proteomics 20210119 1

<h4>Background</h4>Plasma is a potentially rich source of protein biomarkers for disease progression and drug response. Large multi-center studies are often carried out to increase the number of samples analyzed in a given study. This may increase the chances of variation in blood processing and handling, leading to altered proteomic results. This study evaluates the impact of blood processing variation on LC-MS/MS proteomic analysis of plasma.<h4>Methods</h4>Initially two batches of patient pla ...[more]

PMID: 33468058

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