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Construction of Human Proteoform Families from 21 Tesla FT-ICR Mass Spectrometry Top-Down Proteomic Data

ABSTRACT: MCF-7 cell lysate was fractionated using a 10% GELFrEE cartridge. Each fraction was analyzed by 21 T FT-ICR at the National High Magnetic Field Laboratory.

INSTRUMENT(S): 21 Tesla FT-ICR

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lloyd M. Smith

PROVIDER: MSV000085978 | MassIVE | Wed Aug 19 10:58:00 BST 2020

REPOSITORIES: MassIVE

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QE-HF companion data for Construction of Human Proteoform Families from 21 Tesla FT-ICR Mass Spectrometry Top-Down Proteomic Data

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Project description:We present an open-source, interactive program named Proteoform Suite that uses proteoform mass and intensity measurements from complex biological samples to identify and quantify proteoforms. It constructs families of proteoforms derived from the same gene, assesses proteoform function using gene ontology (GO) analysis, and enables visualization of quantified proteoform families and their changes. It is applied here to reveal systemic proteoform variations in the yeast response to salt stress.

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