Proteomics

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Ubiquitylome analysis of Arabidopsis roots.

ABSTRACT: Ubiquitylome analysis of Arabidopsis roots. 10 day old Arabidopsis roots were treated with mock, DMSO, or bortezomib for 3 hours. Root tissue was processed using the phenol-FASP method described in https://doi.org/10.1002/pmic.201800220. Ubiquitin remnant peptides were enriched using an anti-diglycine lysine antibody from PTM-biolabs (PTM-1104). non-enriched as well as di-gly enriched peptides were labeled with TMT10 or TMT16 reagents. Both label-free and TMT labeled peptides were analyzed by LC-MS.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Justin Walley  

PROVIDER: MSV000086156 | MassIVE |

REPOSITORIES: MassIVE

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Publications

Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.

Song Gaoyuan G   Montes Christian C   Olatunji Damilola D   Malik Shikha S   Ji Chonghui C   Clark Natalie M NM   Pu Yunting Y   Kelley Dior R DR   Walley Justin W JW  

The Plant cell 20241201 1

Protein activity, abundance, and stability can be regulated by post-translational modification including ubiquitination. Ubiquitination is conserved among eukaryotes and plays a central role in modulating cellular function; yet, we lack comprehensive catalogs of proteins that are modified by ubiquitin in plants. In this study, we describe an antibody-based approach to enrich ubiquitinated peptides coupled with isobaric labeling to enable quantification of up to 18-multiplexed samples. This appro  ...[more]

PMID: 39570863

Publication: 1/2

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