Proteomics

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Analysis of the Yarrowia lipolytica proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions

ABSTRACT: Y. lipolytica cells were grown in rich-medium and (nutrient limited) minimal-medium, showing accumulations of neutral lipids at 10% and 30% of dry cell weight respectively. To study the mechanisms driving this, we sampled these cultures in paired replicates during both log and stationary phases of the cell growth, and performed 1D LC-MS/MS quantitation analysis on their tryptic digests. Sample assignments were as follows: (RME1, RME2) log phase rich; (RMG3, RMG4) stationary phase rich; (RME10, RME11) log phase minimal; (RME7, RME8) stationary phase minimal. Label free quantitation yielded a log2 expression matrix of over 1800 proteins. Differential analysis showed that while the hypothesized driver of TAG storage- ATP citrate lyase- remained stable, there was up-regulation of proteins involved in the TCA cycle.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Yarrowia Lipolytica (ncbitaxon:4952)

SUBMITTER: David Levin  

PROVIDER: MSV000086249 | MassIVE | Thu Oct 08 08:24:00 BST 2020

REPOSITORIES: MassIVE

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Analysis of the Yarrowia lipolytica proteome reveals subtle variations in expression levels between lipogenic and non-lipogenic conditions.

Sestric Ryan R   Spicer Vic V   Krokhin Oleg V OV   Sparling Richard R   Levin David B DB  

FEMS yeast research 20210301 2

Oleaginous yeasts have the ability to store greater than 20% of their mass as neutral lipids, in the form of triacylglycerides. The ATP citrate lyase is thought to play a key role in triacylglyceride synthesis, but the relationship between expression levels of this and other related enzymes is not well understood in the role of total lipid accumulation conferring the oleaginous phenotype. We conducted comparative proteomic analyses with the oleaginous yeast, Yarrowia lipolytica, grown in either  ...[more]

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