Dataset Information

Secretome dynamics of Streptomyces lividans TK24

ABSTRACT: Proteomic analysis on the secreted proteome of S.lividans TK24 growing in minimal medium (MM), minimal medium supplemented with casamino acids (MMCAS), nutrient broth medium (NB) or phage medium (Ph). Samples were taken in mid-log exponential, late-log exponential and stationary phase.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Streptomyces Lividans Tk24

SUBMITTER: Konstantinos Tsolis

LAB HEAD: Tassos Economou

PROVIDER: PXD009675 | Pride | 2019-02-16

REPOSITORIES: Pride

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Publications

Secretome Dynamics in a Gram-Positive Bacterial Model.

Tsolis Konstantinos C KC Hamed Mohamed Belal MB Simoens Kenneth K Koepff Joachim J Busche Tobias T Rückert Christian C Oldiges Marco M Kalinowski Jörn J Anné Jozef J Kormanec Jan J Bernaerts Kristel K Karamanou Spyridoula S Economou Anastassios A

Molecular & cellular proteomics : MCP 20181129 3

Protein secretion is a central biological process in all organisms. Most studies dissecting bacterial secretion mechanisms have focused on Gram-negative cell envelopes such as that of <i>Escherichia coli</i> However, proteomics analyses in Gram negatives is hampered by their outer membrane. Here we studied protein secretion in the Gram-positive bacterium <i>Streptomyces lividans</i> TK24, in which most of the secretome is released in the growth medium. We monitored changes of the secretome as a ...[more]

PMID: 30498012

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