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A Quantitative Proteome Map of the Human Body

ABSTRACT: In this study, we quantified the relative protein levels from 12,627 genes across 32 normal human tissue types prepared by the GTEx project. Known and new tissue specific or enriched proteins were identified and compared to transcriptome data. Many ubiquitous transcripts are found to encode highly tissue specific proteins. Discordance in the sites of RNA expression and protein detection also revealed potential sites of synthesis and action of protein signaling molecules. Overall, these results provide an extraordinary resource, and demonstrate that understanding protein levels can provide insights into metabolism, regulation, secretome, and human diseases.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: mike snyder

PROVIDER: MSV000086387 | MassIVE | Fri Oct 30 02:18:00 GMT 2020

SECONDARY ACCESSION(S): PXD016999

REPOSITORIES: MassIVE

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A Quantitative Proteome Map of the Human Body.

Jiang Lihua L Wang Meng M Lin Shin S Jian Ruiqi R Li Xiao X Chan Joanne J Dong Guanlan G Fang Huaying H Robinson Aaron E AE Snyder Michael P MP

Cell 20200910 1

Determining protein levels in each tissue and how they compare with RNA levels is important for understanding human biology and disease as well as regulatory processes that control protein levels. We quantified the relative protein levels from over 12,000 genes across 32 normal human tissues. Tissue-specific or tissue-enriched proteins were identified and compared to transcriptome data. Many ubiquitous transcripts are found to encode tissue-specific proteins. Discordance of RNA and protein enric ...[more]

PMID: 32916130

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A Quantitative Proteome Map of the Human Body

Project description:In this study, we quantified the relative protein levels from 12,627 genes across 32 normal human tissue types prepared by the GTEx project. Known and new tissue specific or enriched proteins were identified and compared to transcriptome data. Many ubiquitous transcripts are found to encode highly tissue specific proteins. Discordance in the sites of RNA expression and protein detection also revealed potential sites of synthesis and action of protein signaling molecules. Overall, these results provide an extraordinary resource, and demonstrate that understanding protein levels can provide insights into metabolism, regulation, secretome, and human diseases.

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Map of microbially induced metabolic changes across diverse body sites in mice - Mouse Data

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Project description:Somatic mutations accumulated in normal tissues are associated with aging and disease. Here, we performed a comprehensive genomic analysis of 1,737 morphologically normal tissue biopsies (~ 600 cells each) of nine organs from five donors. We found that somatic mutation accumulations and clonal expansions are widespread, although to variable extents, in morphologically normal human tissues. Somatic copy number alterations were rarely detected, except for tissues from esophagus and cardia. Endogenous mutational processes like SBS1 and SBS5 are ubiquitous among normal tissues though exhibiting different relative activities. Exogenous mutational processes operate in multiple tissues from the same donor. We reconstructed the spatial somatic clonal architecture with sub-millimeter resolution. In esophagus and cardia, macroscopic somatic clones that expanded to hundreds of micrometers were frequently seen, whereas in tissues like colon, rectum, and duodenum, somatic clones were microscopic in size and evolved independently, possibly restricted by local tissue micro-structures. Our study depicted a body map of somatic mutations and clonal expansions from the same individuals, and it revealed that the degree of somatic clonal expansion and enrichment of driver mutations are highly organ specific.

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