Proteomics

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The protein phosphatase PPM1A dephosphorylates and activates YAP to govern mammalian intestinal and liver regeneration


ABSTRACT: Nanoscale liquid chromatography coupled to tandem mass spectrometry analysis for protein identification, characterization and label-free quantification was performed by Phoenix National Proteomics Core. The samples for mass spectrometry analyses were from the in vitro phosphatase assays mentioned above, separated by SDS-PAGE, stained by coomassie blue to locate the YAP, and cut spanning YAP. Pryptic peptides were separated on a C18 column and analyzed by LTQ-Orbitrap Velos (Thermo). Proteins were identified using the search engine of the National Center for Biotechnology Information against the human or mouse RefSeq protein databases.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Pinglong Xu  

PROVIDER: MSV000086694 | MassIVE | Thu Jan 14 00:23:00 GMT 2021

REPOSITORIES: MassIVE

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