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Triazine pyridine chemistry for selective tyrosine labelling of proteins

ABSTRACT: Chemoselective reactions allowing for amino acid-specific modification are essential for protein bioconjugation and covalent drug development. Apart from Lysine and Cysteine with superior nucleophilic side chains, other amino acid-selective reactions are also needed but not well developed. Herein, we report the development of the biocompatible and catalyst-free triazine-pyridine chemistry (TPC) for tyrosine-specific labelling under physiological conditions and profiling in whole proteome. TPC exhibited high tyrosine chemoselectivity in biological systems, displayed potentials in tyrosine-guided protein labelling, and had biocompatibility in living cells. The development of TPC based tyrosine labelling agents would offer additional tools in native protein chemical modification.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Prof. Xuechen Li

PROVIDER: MSV000086762 | MassIVE | Mon Jan 25 03:35:00 GMT 2021

SECONDARY ACCESSION(S): PXD023794

REPOSITORIES: MassIVE

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