Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Triazine pyridine Chemistry for Protein Labelling on Tyrosine.

ABSTRACT: Chemoselective reactions allowing for amino acid-specific modification are essential for protein bioconjugation and covalent drug development. Apart from Lysine and Cysteine with superior nucleophilic side chains, other amino acid-selective reactions are also needed but not well developed. Herein, we report the development of the biocompatible and catalyst-free triazine-pyridine chemistry (TPC) for tyrosine-specific labelling under physiological conditions and profiling in whole proteome. TPC exhibited high tyrosine chemoselectivity in biological systems, displayed potentials in tyrosine-guided protein labelling, and had biocompatibility in living cells. The development of TPC based tyrosine labelling agents would offer additional tools in native protein chemical modification.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Prof. Xuechen Li

PROVIDER: MSV000087968 | MassIVE | Tue Aug 10 23:47:00 BST 2021

SECONDARY ACCESSION(S): PXD027853

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Triazine Pyridine Chemistry for Protein Labelling on Tyrosine

Project description:Chemoselective reactions allowing for amino acid-specific modification are essential for protein bioconjugation and covalent drug development. Apart from Lysine and Cysteine with superior nucleophilic side chains, other amino acid-selective reactions are also needed but not well developed. Herein, we report the development of the biocompatible and catalyst-free triazine-pyridine chemistry (TPC) for tyrosine-specific labelling under physiological conditions and profiling in whole proteome. TPC exhibited high tyrosine chemoselectivity in biological systems, displayed potentials in tyrosine-guided protein labelling, and had biocompatibility in living cells. The development of TPC based tyrosine labelling agents would offer additional tools in native protein chemical modification.

2021-08-10 | MSV000087967 | MassIVE

Triazine pyridine chemistry for selective tyrosine labelling of proteins

2021-01-25 | MSV000086762 | MassIVE

BLU667-adapted transcriptome in CCDC6-RET fusion papillary thyroid carcinoma (TPC) cells

Project description:To investigate the short-termed and long-termed transcriptomic changes induced by the RET tyrosine kinase inhibitor BLU667 in TPC-1 human PTC cells

2023-04-11 | GSE211371 | GEO

Modular oxidation of cytosine modifications and their application in direct and quantitative sequencing of 5-hydroxymethylcytosine

Project description:Selective, efficient, and controllable oxidation of cytosine modifications is valuable for epigenetic analyses, yet only limited progress has been made. Here, we present two modular chemical oxidation reactions: conversion of 5-hydroxymethylcytosine (5hmC) into 5-formylcytosine (5fC) utilizing 4-acetamido-2,2,6,6-tetramethylpiperidine-1-oxoammonium tetrafluoroborate (ACT+ BF4–) and further transformation of 5fC into 5-carboxycytosine (5caC) through Pinnick oxidation. Both reactions are mild and efficient on double-stranded DNA. We integrated these two oxidations with borane reduction to develop chemical-assisted pyridine borane sequencing plus (CAPS+), for direct and quantitative mapping of 5hmC. Compared with chemical-assisted pyridine borane sequencing (CAPS), CAPS+ improved the conversion rate and false-positive rate. We applied CAPS+ to mouse embryonic stem cells (mESCs) DNA, human normal brain and glioblastoma DNA samples, and demonstrated its superior sensitivity in analyzing the hydroxymethylome

2023-03-15 | GSE214006 | GEO

Improved Biocompatibility of Amino-functionalized Graphene Oxide in Caenorhabditis elegans

Project description:Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. Here, we study the adverse effects of GO and amino-functionalized GO (GONH2) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Seq data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. Our work establishes the enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as biomedical nanomaterial.

2022-05-10 | GSE131070 | GEO

Improved Biocompatibility of Amino-functionalized Graphene Oxide in Caenorhabditis elegans

Project description:Improved Biocompatibility of Amino-functionalized Graphene Oxide in Caenorhabditis elegans

| PRJNA542590 | ENA

The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo

Project description:Endocytosis controls the perception of stimuli by modulating protein abundance at the plasma membrane. In plants, clathrin-mediated endocytosis is the most prominent internalization pathway and relies on two multimeric adaptor complexes, the AP-2 and the TPLATE complex (TPC). Ubiquitination is a well-established modification triggering endocytosis of cargo proteins, but how this modification is recognized to initiate the endocytic event remains elusive. Here, we show that TASH3, one of the large subunits of TPC, recognizes ubiquitinated cargo at the plasma membrane via its SH3 domain-containing appendage. TASH3 lacking this evolutionary specific appendage modification allows TPC formation, but the plants show severely reduced endocytic densities, which correlates with reduced endocytic flux. Moreover, comparative plasma membrane proteomics identified differential accumulation of multiple ubiquitinated cargo proteins for which we confirm altered trafficking. Our findings position TPC as a key player for ubiquitinated cargo internalization, allowing future identification of target proteins under specific stress conditions.

2023-03-11 | PXD035444 | Pride

Single-cell mass cytometry reveals the impact of graphene nanomaterials with human primary immune cells

Project description:The genome-wide transcriptome analysis highlight the increased biocompatibility on immune cells of graphene functionalized with amino groups (NH2) compared with graphene oxide (GO); reducing the cell metabolism disfunction. Moreover, GONH2 was found to polarizes T-cell and monocyte activation toward a T helper-1/M1 immune response.

2017-08-13 | GSE99929 | GEO

Hammaren-Geissen2022_PPToP_Model12

Project description:The model encodes the general biological process of protein synthesis and post-translational modification (PTM, such as protein phosphorylation), viewed through the eyes of a specific, widely-used experimental method. Specifically, we model measurements of pulsed stable isotope labelling of amino acids in cell culture (pSILAC), which is a method often used to quantify protein turnover (i.e. the degradation of old and replacement with new) of proteins in a cell.

2024-09-02 | BIOMD0000001078 | BioModels

Project description:Anticodon Modification-based Identification of amiNo acid Overproducers (AMINO)

| PRJNA751045 | ENA