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Comparative proteomic analysis of the three main morphotypes of the diatom Phaeodactylum tricornutum

ABSTRACT: Phaeodactylum tricornutum is an atypical diatom since it can display three main morphotypes: fusiform, triradiate and oval. Such pleiomorphism is possible thanks to an original metabolism, which is tightly regulated in order to acclimate to environmental conditions. Currently, studies dedicated to the comparison of each morphotype issued from one specific strain are scarce and little information is available regarding the physiological significance of this morphogenesis. In this study, we performed a comparative proteomic analysis of intracellular protein extracts and secretomes of the three morphotypes from P. tricornutum. Cultures particularly enriched in one dominant morphotype: fusiform, triradiate or oval of P. tricornutum Pt3 strain were used. Pairwise comparisons highlighted biological processes which are up- and down-regulated in each morphotype as compared to the fusiform one used as a reference.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Phaeodactylum Tricornutum Ccap 1055/1 (ncbitaxon:556484)

SUBMITTER: BARDOR Muriel

PROVIDER: MSV000086835 | MassIVE | Wed Feb 10 10:15:00 GMT 2021

REPOSITORIES: MassIVE

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