Proteomics

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Exhaustive Cell Wall Hydrolase Knockout Bacillus subtilis Strain


ABSTRACT: IMPORTANCE In order to grow, bacterial cells must both create and break down their cell wall. The enzymes that are responsible for these processes are the target of some of our best antibiotics. Our understanding of the proteins that break down the wall - cell wall hydrolases - has been limited by redundancy among the large number of hydrolases many bacteria contain. To solve this problem, we identified 42 cell wall hydrolases in Bacillus subtilis and created a strain lacking 40 of them. We show that cells can survive using only a single cell wall hydrolase; this means that to understand the growth of B. subtilis in standard laboratory conditions, it is only necessary to study a very limited number of proteins, simplifying the problem substantially. We additionally show that the multiple-knockout strain is a research tool to characterize hydrolases, using it to identify 3 "helper" hydrolases that act in certain stress conditions. This submission contains the peptidoglycan profiling dataset (HPLC-MS raw data).

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423)

SUBMITTER: Ethan Garner  

PROVIDER: MSV000086886 | MassIVE | Tue Feb 16 12:01:00 GMT 2021

REPOSITORIES: MassIVE

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