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Alternative polyadenylation quantitative trait loci contributing to complex trait and disease heritability

ABSTRACT: we generated nuclear and cytoplasmic extracts from FLAG-LARP4 cells, purified LARP4, using FLAG affinity resin, and analyzed the purified complexes, using mass spectrometry. Consistent with previous reports, LARP4 is primarily, but not exclusively, cytoplasmic, and we could robustly detect associated proteins involved in poly(A)-binding. Surprisingly, we also detected numerous components of the cleavage and polyadenylation machinery associated with LARP4, suggesting a potential direct role in APA regulation

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: William Russell

PROVIDER: MSV000087000 | MassIVE | Thu Mar 04 15:59:00 GMT 2021

SECONDARY ACCESSION(S): PXD024531

REPOSITORIES: MassIVE

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