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Bound proteins of DNA nanomaterials exposed to human serum

ABSTRACT: DNA aptamers and framework DNA nanostructures are emerging DNA materials with many appealing biological applications including biosensing, bioimaging, drug delivery etc. When placed in physiological fluids, they inevitably encounter biomolecules (majorly proteins) and form complexes that largely affects their biological fate. Nevertheless, little is known regarding the quantitative profile of proteins that adsorb to DNA aptamers and DNA nanostructures in biological environments, and there are no potent strategies to regulate protein profiles. Herein, we performed a proteomic analysis to profile proteins that bind to DNA aptamers (Sgc8c and SYLC3) and nanostructures (a tetrahedral DNA nanostructure and a DNA origami rod) in human serum by using liquid chromatography mass spectrometry (LC-MS).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Pengfei Wang

PROVIDER: MSV000087433 | MassIVE | Sat May 15 04:05:00 BST 2021

SECONDARY ACCESSION(S): PXD026025

REPOSITORIES: MassIVE

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