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Overexpression of griseusins in Streptomyces sp. CA-256286

ABSTRACT: Employing multi-omics methods for overexpression of griseusins in Streptomyces sp. CA-25628. This set of data proves that the introduction of the SARPs regulator activates the production of griseusins ( [M+H]+= 749.2589, 586.2288, 796.2771), the inactivation of the griseusin expression using CRISPR-cBEST base editing, and the heterologous expression of the BGC responsible for their production, complemented with the SARPs regulator, activates their production in S. albus J1074.

INSTRUMENT(S): 6545 Q-TOF LC/MS, Orbitrap Fusion

ORGANISM(S): Streptomyces Sp. Ca-256286 Streptomyces Albus J1074 (ncbitaxon:457425)

SUBMITTER: Karl Tilmann Weber

PROVIDER: MSV000087475 | MassIVE | Fri May 21 05:41:00 BST 2021

REPOSITORIES: MassIVE

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Publications

Complete Genome Sequence of <i>Streptomyces</i> sp. Strain CA-256286.

Beck Charlotte C Gren Tetiana T Jørgensen Tue S TS González Ignacio I Román-Hurtado Fernando F Oves-Costales Daniel D Genilloud Olga O Weber Tilmann T

Microbiology resource announcements 20210603 22

Here, we report the sequencing, assembly, and annotation of the genome of <i>Streptomyces</i> sp. strain CA-256286. The genome consists of a linear 7,726,360-nucleotide chromosome and a linear 466,817-nucleotide putative plasmid. This strain is predicted to produce a range of novel secondary metabolites. ...[more]

PMID: 34080908

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