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Gene expression profile of M.tuberculosis espR (Rv3849) mutant complemented with an N-terminal Flag espR gene

ABSTRACT: Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of four M.tb strains grown in log phase: Erdman strain of M.tb, espR transposon mutant, the espR transposon mutant complemented with the wild type copy of the espR gene, and the espR transposon mutant complemented with an N-terminal FLAG espR gene.

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Paolo Manzanillo

PROVIDER: E-GEOD-12379 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Secreted transcription factor controls Mycobacterium tuberculosis virulence.

Raghavan Sridharan S Manzanillo Paolo P Chan Kaman K Dovey Cole C Cox Jeffery S JS

Nature 20080801 7205

Bacterial pathogens trigger specialized virulence factor secretion systems on encountering host cells. The ESX-1 protein secretion system of Mycobacterium tuberculosis-the causative agent of the human disease tuberculosis-delivers bacterial proteins into host cells during infection and is critical for virulence, but how it is regulated is unknown. Here we show that EspR (also known as Rv3849) is a key regulator of ESX-1 that is required for secretion and virulence in mice. EspR activates transcr ...[more]

PMID: 18685700

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Gene expression profile of M.tuberculosis espR (Rv3849) mutants containing N- or C-terminal mutations in the espR gene

Project description:Mycobacterium tuberculosis, a pathogen of global importance, utilizes the ESX-1 protein secretion system to export virulence factors that disarm host macrophages. Although this secretory pathway is critical for virulence, how ESX-1 is regulated is completely unknown. Here we show that EspR (Rv3849) is a key regulator of ESX-1. EspR activates transcription of an operon that includes three ESX-1 components, Rv3616c-Rv3614c, whose expression in turn promotes secretion of ESX-1 substrates. Keywords: Strain comparison Comparison of the global gene expression of the following M.tb strains grown in log phase: Erdman strain of M.tb (WT), espR transposon mutant (R-), the espR transposon mutant complemented with the espR gene (R+), the espR mutant (R-) complemented with an espR gene containing an R8A amino acid subsitution (R- R8A), the espR mutant (R-) complemented with an espR gene containing an R21A amino acid subsitution (R- R21A), espR mutant (R-) complemented with an espR gene containing a C-terminal 10-amino acid truncation (R- delta10), and the espR mutant (R-) complemented with an espR gene containing a C-terminal 5-amino acid truncation (R- delta5).

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