Proteomic analysis of tropomyosin isoforms expressed and purified from Expi293F (human) cells.
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ABSTRACT: Human tropomyosin (Tpm) isoforms (Tpm1.6, Tpm1.7, Tpm2.1, Tpm3.1, Tpm3.2, Tpm4.2) and alpha-synuclein were expressed in Expi293F (human) cells as fusion proteins with a C-terminal intein + chitin binding domain tag. After purification on chitin resin, self-cleavage of the intein released the target proteins. Each purified protein was then analyzed by mass spectrometry.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER:
Roberto Dominguez
PROVIDER: MSV000087517 | MassIVE | Tue May 25 21:20:00 BST 2021
REPOSITORIES: MassIVE
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