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Quantitative proteomic atlas of soybean-rhizobium symbiotic interface

ABSTRACT: An effective symbiosis between legumes and rhizobia relies largely on the diverse proteins on the plant-rhizobium interface, the symbiosome membrane (SM) and peribacteroid space (PBS), for material transportation and signal transduction during symbiotic nitrogen fixation. Employing higher resolution, faster scanning speed, and higher sensitivity LC-MS/MS technique, combining with label-free quantitative proteomic approaches, here we identified more than a thousand soybean proteins and hundreds of rhizobia proteins for either SM or PBS.

INSTRUMENT(S): Q Exactive mass spectrometer (Thermo Scientific)

ORGANISM(S): Glycine Max (ncbitaxon:3847) Bradyrhizobium Japonicum Usda 110 (ncbitaxon:224911)

SUBMITTER: Wei-Cai Yang

PROVIDER: MSV000087542 | MassIVE | Fri May 28 19:59:00 BST 2021

SECONDARY ACCESSION(S): PXD026357

REPOSITORIES: MassIVE

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Publications

Quantitative proteomics reveals key pathways in the symbiotic interface and the likely extracellular property of soybean symbiosome.

Luo Yu Y Liu Wei W Sun Juan J Zhang Zheng-Rong ZR Yang Wei-Cai WC

Journal of genetics and genomics = Yi chuan xue bao 20220422 1

An effective symbiosis between legumes and rhizobia relies largely on diverse proteins at the plant-rhizobium interface for material transportation and signal transduction during symbiotic nitrogen fixation. Here, we report a comprehensive proteome atlas of the soybean symbiosome membrane (SM), peribacteroid space (PBS), and root microsomal fraction (RMF) using state-of-the-art label-free quantitative proteomic technology. In total, 1759 soybean proteins with diverse functions are detected in th ...[more]

PMID: 35470091

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