Dataset Information

Soybean Symbiosome Membrane Proteome

ABSTRACT: Symbiosome membranes (SM) and symbiosome space (SS) fractions were isolated from nitrogen fixing soybean root nodules and analyzed using non-gel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to enrich for hydrophobic integral membrane proteins.. One-hundred and seventy-two proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and SS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and targeting, signalling and transport were identified. These included a number of proteins previously localized to the SM, such as nodulin 26, an aquaglyceroporin. Transport classes identified include sulphate, calcium, peptide/dicarboxylate, nitrate and metal ion transporters. This proteome will provide a rich resource for the study of the legume-rhizobium symbiosis.

INSTRUMENT(S): QSTAR

ORGANISM(S): Glycine Max

TISSUE(S): Root Nodule

SUBMITTER: Victoria Clarke

LAB HEAD: Penny Smith

PROVIDER: PXD001132 | Pride | 2015-08-12

REPOSITORIES: Pride

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			Action	DRS
	20110827_J1D (J1D).mzid_20110827_J1D_(J1D).pride.mgf.gz	Mzid
	20110827_J1D (J1D).pride.mztab.gz	Mztab
	20110827_J1DJ1D.mzid.gz	Mzid
	20110827_J1DJ1D.mzid_20110827_J1D_J1D.MGF	Mzid
	20110827_J1_A (J1A).mzid_20110827_J1_A_(J1A).pride.mgf.gz	Mzid

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Proteomic analysis of the soybean symbiosome identifies new symbiotic proteins.

Clarke Victoria C VC Loughlin Patrick C PC Gavrin Aleksandr A Chen Chi C Brear Ella M EM Day David A DA Smith Penelope M C PM

Molecular & cellular proteomics : MCP 20150227 5

Legumes form a symbiosis with rhizobia in which the plant provides an energy source to the rhizobia bacteria that it uses to fix atmospheric nitrogen. This nitrogen is provided to the legume plant, allowing it to grow without the addition of nitrogen fertilizer. As part of the symbiosis, the bacteria in the infected cells of a new root organ, the nodule, are surrounded by a plant-derived membrane, the symbiosome membrane, which becomes the interface between the symbionts. Fractions containing th ...[more]

PMID: 25724908

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