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Quantification on NOD1 ubiquitination sites with ubiquitin and SspH2 co-overexpression

ABSTRACT: HEK293T cells were transiently transfected with NOD1-FLAG, HA-SspH2 WT/HA-SspH2 C580/empty vector, and Myc-Ubiquitin. Following anti-FLAG automated immunoprecipitation and in-gel trypsin digestion, fractionated samples were injected onto LC-MS/MS. Quantitation was performed on diglycyl-lysine sites on NOD1, on identical peptides with highest intensity across samples.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Olivier Julien Amit Bhavsar

PROVIDER: MSV000087693 | MassIVE | Wed Jun 23 16:43:00 BST 2021

REPOSITORIES: MassIVE

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Quantification on NOD1 ubiquitination sites with SspH2 co-overexpression

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