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Deciphering the ciliary extracellular vesicle (EV) proteome

ABSTRACT: Cilia are conserved EV shedding organelles that release ciliary EVs for transmitting 13 signals. However, the content of ciliary EVs produced by living animals has not been explored. 14 Here we used evolutionarily conserved PKD-2::GFP EV cargo of Caenorhabditis elegans as a 15 marker of ciliary EVs to identify animal EV proteome. Top candidates were experimentally 16 confirmed using fluorescent reporter tracking and live animal imaging. Our findings indicate that 17 cilia produce multiple EVs with distinct protein content and that EVs from different neuronal 18 types carry different EV cargo. We discover that ciliary EVs carry nucleic acid binding proteins, 19 implying the role of cilia in epigenetic regulation and transfer of genetic information in trans. 20 Our dataset also contains non-ciliary EV cargo candidates from other tissues, such as cuticle, 21 somatic gonad, and germline, that can be explored using our MyEVome Web App.

INSTRUMENT(S): Orbitrap Eclipse, Q Exactive HF

ORGANISM(S): Escherichia Coli (ncbitaxon:562) Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Maureen M Barr

PROVIDER: MSV000087943 | MassIVE | Wed Aug 04 10:50:00 BST 2021

REPOSITORIES: MassIVE

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