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Lung cancer plasma glycoproteome correlation with plasma DSC thermograms

ABSTRACT: Blood plasma from patients diagnosed with different subtypes of lung cancer or subjects with benign nodules was analyzed by using isobaric labeling and correlating to plasma thermograms. Plasma samples were digested using a FASP approach, labeled with TMT11plex reagents, admixed, deglycosylated using NEB Deglycoslylation Mix II in a non-denaturing approach. Deglycosylated peptides were eluted and analyzed by 1DLC-(low pH RP C-18)-MS. Collected data were analyzed by PD 2.1 using SequestHT and Mascot considering N-linked de-glycosylation of plasma proteins with Asn>Asp and Gln>Glu conversions along with standard modifications (TMT, Met(ox), Cys(CAM)) using an Orbitrap ELITE mass spectrometer. TMT reporter ion values were normalized to pooled internal standard TMT131 label and median glycopeptide abundance values of selected proteins were compared between control subjects and LC patients.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael L. Merchant, PhD

PROVIDER: MSV000088001 | MassIVE | Wed Aug 18 11:38:00 BST 2021

REPOSITORIES: MassIVE

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