Project description:Differential analysis of proteomes originated from early lesion of colorectal cancer and colon control tissue using FFPE-FASPTM and Label free analysis using 2DnanoUPLC separation before analysis on a qTOF synapt HDMS G2 using ion mobility as supplementary separation dimension
Project description:Untargeted bottom-up whole proteome analysis of EA-cells expressing cytosolic HyPer7 biosensor using ACQUITY UPLC M-Class, coupled to a SYNAPT G2-Si MS
Project description:Optimising the parameters for cross-link analysis using a QToF mass spectrometer. We performed triplicate analysis of 6 different collision energy ramps to determine the optimal energy for cross-link fragmentation.
Project description:Proteomics and phosphoproteomics analysis of MCF7 cells sensitive and resistant to the PI3K inhibitor GDC-0941. The analysis was done for parental and 3 resistant cell lines maintained in the presence or the absence of the drug. One of the resistant cell lines (G2) was also treated with vehicle or the kinase inhibitors GDC0941, MK-2206 and Ku-0063794 for 2h. The proteomics analysis of sensitive and resistant MCF7 cells in basal conditions and phosphoproteomics analysis of the G2 cells in the presence of inhibitors of the PI3K pathway were run in a nano flow ultrahigh pressure liquid chromatography (UPLC, nano Acquity, Waters) coupled to an LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific). The phosphoproteomics study of sensitive and resistant MCF7 cells in basal conditions was run in a Dionex UltiMate 3000 RSLCnano coupled to an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific).