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QToF Cross-linking Optimisation

ABSTRACT: Optimising the parameters for cross-link analysis using a QToF mass spectrometer. We performed triplicate analysis of 6 different collision energy ramps to determine the optimal energy for cross-link fragmentation.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Juliette James

LAB HEAD: Konstantinos Thalassinos

PROVIDER: PXD011704 | Pride | 2019-11-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	5ppm10ppm_fbsaSTYK_150814_bs3d0d12_dda_hdramp3_ss_intra.csv	Csv
	5ppm10ppm_fbsaSTYK_160525_bs3d0d12_dda_low1_ss_intra.csv	Csv
	5ppm10ppm_fbsaSTYK_160525_bs3d0d12_dda_low2_ss_intra.csv	Csv
	5ppm10ppm_fbsaSTYK_160615_bs3d0d12_dda_hditraq3_ss_intra.csv	Csv
	5ppm10ppm_fbsaSTYK_161103_bs3d0d12_dda_hditraq2_ss_intra.csv	Csv

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Publications

Optimization Workflow for the Analysis of Cross-Linked Peptides Using a Quadrupole Time-of-Flight Mass Spectrometer.

James Juliette M B JMB Cryar Adam A Thalassinos Konstantinos K

Analytical chemistry 20190123 3

Cross-linking mass spectrometry is an emerging structural biology technique. Almost exclusively, the analyzer of choice for such an experiment has been the Orbitrap. We present an optimized protocol for the use of a Synapt G2-Si for the analysis of cross-linked peptides. We first tested six different energy ramps and analyzed the fragmentation behavior of cross-linked peptides identified by xQuest. By combining the most successful energy ramps, cross-link yield can be increased by up to 40%. Whe ...[more]

PMID: 30620560

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eXL-MS: An enhanced Cross-Linking Mass Spectrometry Workflow to Study Protein Complexes.

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