The 2020 ABRF Beer Study: beer proteomics at the global scale

ABSTRACT: Beer is one of the oldest and most widely consumed beverages in the world. It contains a complex mixture of proteins from several organisms including plants (barley, hops, rice, wheat) and yeast, depending on the beer. It has been argued that fermentation of grains was responsible for our shift away from being a hunter-gatherer society, leading to social and technological advancements over thousands of years, including mass spec-based proteomics. The Proteomics Research Group (PRG) in the Association of Biomolecular Resource Facilities brought together an international consortium of proteomics laboratories (69 laboratories from 33 countries) to perform beer proteomics. An aliquot of specially brewed beer from the UC Davis Brewing Program was sent to 52 labs, and participants were encouraged to also use a widely available commercial beer (Heineken) and any other beer. Of the 69 laboratories that signed up for the study, 35 returned data. Of these, 32 analyzed the PRG Beer and 17 analyzed Heineken (14 analyzed both). When shipping of the PRG Beer was prohibitive, participants were encouraged to use Heineken due to its global availability and perceived quality control. Participants were also encouraged to analyze other beers of their choosing, and 79 other beers were analyzed. In total there were 357 beer injections on 13 different types of mass spectrometers around the world. In addition to helping connect scientists in trying times, we have also demonstrated the utility of multi-center studies with accessible materials.

INSTRUMENT(S): Orbitrap Eclipse, Orbitrap Fusion Lumos, timsTOF Pro, Exactive Plus, TripleTOF 5600+, TripleTOF 6600, LTQ Orbitrap XL, Orbitrap Fusion, Orbitrap Exploris 480, Q Exactive HF, Q Exactive

ORGANISM(S): Triticum Aestivum (ncbitaxon:4565) Hordeum Vulgare (ncbitaxon:4513) Oryza Sativa (ncbitaxon:4530) Saccharomyces Cerevisiae (ncbitaxon:4932) Humulus Lupulus (ncbitaxon:3486)

SUBMITTER: Benjamin Neely   Brett Phinney  

PROVIDER: MSV000088080 | MassIVE |

REPOSITORIES: MassIVE